Isolation and characterization of hemolytic genes from Actinobacillus actinomycetemcomitans.
نویسندگان
چکیده
Periodontopathic Actinobacillus actinomyceremcomitans produces hemolysin and other leukotoxins. In the present study, two distinct clones which lysed horse erythrocytes were isolated by screening genomic DNA libraries of A. actinomycetemcomitans ATCC 43718 on blood agar plates. DNA hybridization analysis indicates that there were two distinct hemolytic genes present. Sonicated extracts from both Escherichia coli clones possessed hemolytic activities on horse, sheep and human erythrocytes, but not those of rabbit. Rabbit antiserum to A. actinomycetemcomitans ATCC 43718 whole cells inhibited the hemolytic activities of these clones.
منابع مشابه
Evolutionary lineages of Actinobacillus actinomycetemcomitans bear diverse traits to support roles as a member of normal flora as a pathogen
8 REVIEW OF THE LITERATURE 10 1. Periodontitis 10 1.1 General characteristics and classification 10 1.2 Oral biofilms 10 1.3 Actinobacillus actinomycetemcomitans in periodontitis 12 2. Evolutionary lineages of A. actinomycetemcomitans 14 2.1 Population structure 14 2.2 Natural transformation 16 2.3 Intraspecies diversity 17 2.4 Serotypes and serotype-specific antigens 19 2.5 Serotypes vs. genot...
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عنوان ژورنال:
- FEMS microbiology letters
دوره 143 2-3 شماره
صفحات -
تاریخ انتشار 1996